Dating aroclors new englane
Human sperm exposed to continuous Aroclor 1254 exhibited: (i) reduced sperm motility and kinematic parameters, (ii) a proportion of sperm with high ΔΨm that decreased in a dose-dependent manner (P How to cite this article: Jiang LG, Cheng LY, Kong SH, Yang Y, Shen YJ, Chen C, Deng XH, Liu SZ, Chao L.Toxic effects of polychlorinated biphenyls (Aroclor 1254) on human sperm motility.The study was approved by the Ethics Committee of Qilu Hospital at Shandong University, and there is no conflict of interest.Donors were informed about the purpose of the study and asked for their consent.As a result of their lipophilicity and chemical stability, PCBs have been detected all over the world in remote regions, PCBs have attracted considerable attention due to their ability to concentrate in food chains and their persistence in organisms.During the past few decades, there has been a decline of organism PCBs levels associated with the production ban on PCBs.Sperm preparation and Aroclor 1254 treatment Stock solutions of Aroclor 1254 at 50 mg l at the time of treatment.
Semen samples were collected by masturbation after a recommended sexual abstinence of 3-5 days.
As a control, sperm suspensions were diluted with 0.3% (v/v) olive oil.
Sperm were collected by density-gradient preparation and prepared as described previously. The samples were centrifuged, and motile sperm were resuspended in G-mops containing Aroclor 1254 at different concentrations.
Ten thousand sperm from each group were used in the evaluation of ΔΨm.
Intracellular ROS production DCFH-DA, an oxidation-sensitive fluorescent probe, was used to analyze ROS generation. The cellular mean fluorescence intensity (MFI) was measured using flow cytometry.