Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme.The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (Pub Med:12699694).By in situ hybridization, Localization of the gene encoding peptidylglycine alpha-amidating monooxygenase (PAM) to human chromosome 5q14-5q21.Genomics 18: 319-321, 1993.[Pub Med: 8288234] [Full Text]" pmid="8288234"Ouafik et al. They noted that the NEC1 gene (162150), which encodes another enzyme important in posttranslational processing of neuroendocrine precursors, prohormone convertase 1 (PC1), is located in the same region (5q15-q21).Peptidylglycine alpha-amidating monooxygenase (EC 22.214.171.124) is a multifunctional protein containing 2 enzymes, peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL), that act sequentially to catalyze the alpha-amidation of neuroendocrine peptides. Localization of the gene encoding peptidylglycine alpha-amidating monooxygenase (PAM) to human chromosome 5q14-5q21.Human peptidylglycine alpha-amidating monooxygenase: c DNA, cloning and functional expression of a truncated form in COS cells. Genomics 18: 319-321, 1993.[Pub Med: 8288234] [Full Text]" pmid="8288234"Ouafik et al.It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first. The checksum is a form of redundancy check that is calculated from the sequence. It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low. These are stable identifiers and should be used to cite Uni Prot KB entries.
Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’. This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein.
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Using Kalirin conditional knockout mice to distinguish its role in dopamine receptor mediated behaviors.
The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (Pub Med:12699694).
Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (By similarity).